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Effect of Tax on gene expression of <t>the</t> <t>JNK</t> pathway in Cd-induced apoptosis of chicken primary kidney cells. (A) Molecular docking results between Tax and JNK. (B) Cellular Thermal Shift Assay to verify the interaction between Tax and JNK. (C) Relative protein expression of <t>p-JNK/JNK</t> and relative mRNA expression of JNK. * Indicates a significant difference between DMSO group and Cd group (n=10; * P < 0.05). # Indicates a significant difference between Cd + Tax group and Cd group ( n = 10; # P < 0.05). Results are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA or unpaired Student’s t-test.
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p jnk rabbit ab  (Abmart Inc)
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Effect of Tax on gene expression of <t>the</t> <t>JNK</t> pathway in Cd-induced apoptosis of chicken primary kidney cells. (A) Molecular docking results between Tax and JNK. (B) Cellular Thermal Shift Assay to verify the interaction between Tax and JNK. (C) Relative protein expression of <t>p-JNK/JNK</t> and relative mRNA expression of JNK. * Indicates a significant difference between DMSO group and Cd group (n=10; * P < 0.05). # Indicates a significant difference between Cd + Tax group and Cd group ( n = 10; # P < 0.05). Results are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA or unpaired Student’s t-test.
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p jnk t40074 antibodies  (Abmart Inc)
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Effect of Tax on gene expression of <t>the</t> <t>JNK</t> pathway in Cd-induced apoptosis of chicken primary kidney cells. (A) Molecular docking results between Tax and JNK. (B) Cellular Thermal Shift Assay to verify the interaction between Tax and JNK. (C) Relative protein expression of <t>p-JNK/JNK</t> and relative mRNA expression of JNK. * Indicates a significant difference between DMSO group and Cd group (n=10; * P < 0.05). # Indicates a significant difference between Cd + Tax group and Cd group ( n = 10; # P < 0.05). Results are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA or unpaired Student’s t-test.
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p jnk fy er5754 118 elisa kit  (Wuhan Feiyue Biotechnology Co Ltd)
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Effect of Tax on gene expression of <t>the</t> <t>JNK</t> pathway in Cd-induced apoptosis of chicken primary kidney cells. (A) Molecular docking results between Tax and JNK. (B) Cellular Thermal Shift Assay to verify the interaction between Tax and JNK. (C) Relative protein expression of <t>p-JNK/JNK</t> and relative mRNA expression of JNK. * Indicates a significant difference between DMSO group and Cd group (n=10; * P < 0.05). # Indicates a significant difference between Cd + Tax group and Cd group ( n = 10; # P < 0.05). Results are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA or unpaired Student’s t-test.
P Jnk Fy Er5754 118 Elisa Kit, supplied by Wuhan Feiyue Biotechnology Co Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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p jnk  (Affinity Biosciences)
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Effect of Tax on gene expression of <t>the</t> <t>JNK</t> pathway in Cd-induced apoptosis of chicken primary kidney cells. (A) Molecular docking results between Tax and JNK. (B) Cellular Thermal Shift Assay to verify the interaction between Tax and JNK. (C) Relative protein expression of <t>p-JNK/JNK</t> and relative mRNA expression of JNK. * Indicates a significant difference between DMSO group and Cd group (n=10; * P < 0.05). # Indicates a significant difference between Cd + Tax group and Cd group ( n = 10; # P < 0.05). Results are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA or unpaired Student’s t-test.
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p jnk1 2 3  (Huabio Inc)
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Effect of Tax on gene expression of <t>the</t> <t>JNK</t> pathway in Cd-induced apoptosis of chicken primary kidney cells. (A) Molecular docking results between Tax and JNK. (B) Cellular Thermal Shift Assay to verify the interaction between Tax and JNK. (C) Relative protein expression of <t>p-JNK/JNK</t> and relative mRNA expression of JNK. * Indicates a significant difference between DMSO group and Cd group (n=10; * P < 0.05). # Indicates a significant difference between Cd + Tax group and Cd group ( n = 10; # P < 0.05). Results are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA or unpaired Student’s t-test.
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p jnk  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc p jnk
Knocking down SKA2-induced cell-cycle arrest and apoptosis through the SKA2/ROS/ATM axis in GC cell lines (A) Western blotting analysis of γ-H2AX (Ser139), ATM, p -ATM (Ser1981), p -Chk2 (Thr68), and SKA2 expression in SNU638 and NUGC3 SKA2-knockdown cell lines. (B) Western blotting analysis of SKA2 overexpression in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against γ-H2AX (Ser139), ATM, p -ATM (Ser1981), p -Chk2 (Thr68), and SKA2. (C) Western blotting analysis of KU-55933 treatment in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against cyclin D1, cyclin A2, cyclin B1, p -Chk2 (Thr68), PARP, <t>Cleaved-Caspase3,</t> <t>JNK,</t> p -JNK (Thr183/Tyr185), ATM, p -ATM (Ser1981), and SKA2. (D) Western blotting analysis of BML-277 treatment in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against cyclin D1, cyclin A2, cyclin B1, p -Chk2 (Thr68), and SKA2. (E) Western blotting analysis of P38, p-P38 (Thr180/Tyr182), ERK, p -ERK1/2 (Thr202/Tyr204), JNK, p -JNK (Thr183/Tyr185), and SKA2 expression in SNU638 and NUGC3 SKA2-knockdown cell lines. (F) Western blotting analysis of the rescue effect of SKA2 overexpression on MAPK pathway markers (ERK, p -ERK1/2, JNK, and p -JNK) in SNU638 and NUGC3 SKA2-knockdown cell lines. (G) Western blotting analysis of JNK-IN-8 treatment in SNU638 SKA2-knockdown cell lines using antibodies against PARP, cleaved-caspase3, JNK, p -JNK (Thr183/Tyr185), and SKA2. α-Tubulin was used as the internal control for all blots. Representative blotting images are shown from 3 independent experiments.
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rabbit anti p jnk  (Bioss)
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Knocking down SKA2-induced cell-cycle arrest and apoptosis through the SKA2/ROS/ATM axis in GC cell lines (A) Western blotting analysis of γ-H2AX (Ser139), ATM, p -ATM (Ser1981), p -Chk2 (Thr68), and SKA2 expression in SNU638 and NUGC3 SKA2-knockdown cell lines. (B) Western blotting analysis of SKA2 overexpression in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against γ-H2AX (Ser139), ATM, p -ATM (Ser1981), p -Chk2 (Thr68), and SKA2. (C) Western blotting analysis of KU-55933 treatment in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against cyclin D1, cyclin A2, cyclin B1, p -Chk2 (Thr68), PARP, <t>Cleaved-Caspase3,</t> <t>JNK,</t> p -JNK (Thr183/Tyr185), ATM, p -ATM (Ser1981), and SKA2. (D) Western blotting analysis of BML-277 treatment in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against cyclin D1, cyclin A2, cyclin B1, p -Chk2 (Thr68), and SKA2. (E) Western blotting analysis of P38, p-P38 (Thr180/Tyr182), ERK, p -ERK1/2 (Thr202/Tyr204), JNK, p -JNK (Thr183/Tyr185), and SKA2 expression in SNU638 and NUGC3 SKA2-knockdown cell lines. (F) Western blotting analysis of the rescue effect of SKA2 overexpression on MAPK pathway markers (ERK, p -ERK1/2, JNK, and p -JNK) in SNU638 and NUGC3 SKA2-knockdown cell lines. (G) Western blotting analysis of JNK-IN-8 treatment in SNU638 SKA2-knockdown cell lines using antibodies against PARP, cleaved-caspase3, JNK, p -JNK (Thr183/Tyr185), and SKA2. α-Tubulin was used as the internal control for all blots. Representative blotting images are shown from 3 independent experiments.
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Image Search Results


Effect of Tax on gene expression of the JNK pathway in Cd-induced apoptosis of chicken primary kidney cells. (A) Molecular docking results between Tax and JNK. (B) Cellular Thermal Shift Assay to verify the interaction between Tax and JNK. (C) Relative protein expression of p-JNK/JNK and relative mRNA expression of JNK. * Indicates a significant difference between DMSO group and Cd group (n=10; * P < 0.05). # Indicates a significant difference between Cd + Tax group and Cd group ( n = 10; # P < 0.05). Results are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA or unpaired Student’s t-test.

Journal: Poultry Science

Article Title: Cadmium-induced apoptosis via ROS/JNK pathway in chicken primary kidney cells and antagonism of taxifolin

doi: 10.1016/j.psj.2026.106920

Figure Lengend Snippet: Effect of Tax on gene expression of the JNK pathway in Cd-induced apoptosis of chicken primary kidney cells. (A) Molecular docking results between Tax and JNK. (B) Cellular Thermal Shift Assay to verify the interaction between Tax and JNK. (C) Relative protein expression of p-JNK/JNK and relative mRNA expression of JNK. * Indicates a significant difference between DMSO group and Cd group (n=10; * P < 0.05). # Indicates a significant difference between Cd + Tax group and Cd group ( n = 10; # P < 0.05). Results are presented as mean ± SEM. Statistical significance was determined by one-way ANOVA or unpaired Student’s t-test.

Article Snippet: p-JNK , Wanleibio , 1:500 , WL01813.

Techniques: Gene Expression, Thermal Shift Assay, Expressing

The protein levels of cells treated with SP600125 and NAC. (A) Protein expression of p-JNK/JNK, Bad, Bid, Bcl-2, Bax, Bak, DIABLO, XIAP, Caspase-3, and Caspase-9. (B) Quantitative analysis of p-JNK/JNK, Bad, Bid, Bcl-2, Bax, Bak, DIABLO, XIAP, Caspase-3, and Caspase-9 expression. Data are presented as mean ± SD (n = 10). * Indicates a significant difference on Cd + SP600125 or Cd + NAC vs Cd group ( n = 10; * P < 0.05). # Indicates a significant difference between Cd + SP600125 + Tax group and Cd + Tax group ( n = 10; # P < 0.05).

Journal: Poultry Science

Article Title: Cadmium-induced apoptosis via ROS/JNK pathway in chicken primary kidney cells and antagonism of taxifolin

doi: 10.1016/j.psj.2026.106920

Figure Lengend Snippet: The protein levels of cells treated with SP600125 and NAC. (A) Protein expression of p-JNK/JNK, Bad, Bid, Bcl-2, Bax, Bak, DIABLO, XIAP, Caspase-3, and Caspase-9. (B) Quantitative analysis of p-JNK/JNK, Bad, Bid, Bcl-2, Bax, Bak, DIABLO, XIAP, Caspase-3, and Caspase-9 expression. Data are presented as mean ± SD (n = 10). * Indicates a significant difference on Cd + SP600125 or Cd + NAC vs Cd group ( n = 10; * P < 0.05). # Indicates a significant difference between Cd + SP600125 + Tax group and Cd + Tax group ( n = 10; # P < 0.05).

Article Snippet: p-JNK , Wanleibio , 1:500 , WL01813.

Techniques: Expressing

Knocking down SKA2-induced cell-cycle arrest and apoptosis through the SKA2/ROS/ATM axis in GC cell lines (A) Western blotting analysis of γ-H2AX (Ser139), ATM, p -ATM (Ser1981), p -Chk2 (Thr68), and SKA2 expression in SNU638 and NUGC3 SKA2-knockdown cell lines. (B) Western blotting analysis of SKA2 overexpression in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against γ-H2AX (Ser139), ATM, p -ATM (Ser1981), p -Chk2 (Thr68), and SKA2. (C) Western blotting analysis of KU-55933 treatment in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against cyclin D1, cyclin A2, cyclin B1, p -Chk2 (Thr68), PARP, Cleaved-Caspase3, JNK, p -JNK (Thr183/Tyr185), ATM, p -ATM (Ser1981), and SKA2. (D) Western blotting analysis of BML-277 treatment in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against cyclin D1, cyclin A2, cyclin B1, p -Chk2 (Thr68), and SKA2. (E) Western blotting analysis of P38, p-P38 (Thr180/Tyr182), ERK, p -ERK1/2 (Thr202/Tyr204), JNK, p -JNK (Thr183/Tyr185), and SKA2 expression in SNU638 and NUGC3 SKA2-knockdown cell lines. (F) Western blotting analysis of the rescue effect of SKA2 overexpression on MAPK pathway markers (ERK, p -ERK1/2, JNK, and p -JNK) in SNU638 and NUGC3 SKA2-knockdown cell lines. (G) Western blotting analysis of JNK-IN-8 treatment in SNU638 SKA2-knockdown cell lines using antibodies against PARP, cleaved-caspase3, JNK, p -JNK (Thr183/Tyr185), and SKA2. α-Tubulin was used as the internal control for all blots. Representative blotting images are shown from 3 independent experiments.

Journal: iScience

Article Title: SKA2 promotes gastric cancer progression by regulating glutathione metabolism

doi: 10.1016/j.isci.2026.115202

Figure Lengend Snippet: Knocking down SKA2-induced cell-cycle arrest and apoptosis through the SKA2/ROS/ATM axis in GC cell lines (A) Western blotting analysis of γ-H2AX (Ser139), ATM, p -ATM (Ser1981), p -Chk2 (Thr68), and SKA2 expression in SNU638 and NUGC3 SKA2-knockdown cell lines. (B) Western blotting analysis of SKA2 overexpression in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against γ-H2AX (Ser139), ATM, p -ATM (Ser1981), p -Chk2 (Thr68), and SKA2. (C) Western blotting analysis of KU-55933 treatment in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against cyclin D1, cyclin A2, cyclin B1, p -Chk2 (Thr68), PARP, Cleaved-Caspase3, JNK, p -JNK (Thr183/Tyr185), ATM, p -ATM (Ser1981), and SKA2. (D) Western blotting analysis of BML-277 treatment in SNU638 and NUGC3 SKA2-knockdown cell lines using antibodies against cyclin D1, cyclin A2, cyclin B1, p -Chk2 (Thr68), and SKA2. (E) Western blotting analysis of P38, p-P38 (Thr180/Tyr182), ERK, p -ERK1/2 (Thr202/Tyr204), JNK, p -JNK (Thr183/Tyr185), and SKA2 expression in SNU638 and NUGC3 SKA2-knockdown cell lines. (F) Western blotting analysis of the rescue effect of SKA2 overexpression on MAPK pathway markers (ERK, p -ERK1/2, JNK, and p -JNK) in SNU638 and NUGC3 SKA2-knockdown cell lines. (G) Western blotting analysis of JNK-IN-8 treatment in SNU638 SKA2-knockdown cell lines using antibodies against PARP, cleaved-caspase3, JNK, p -JNK (Thr183/Tyr185), and SKA2. α-Tubulin was used as the internal control for all blots. Representative blotting images are shown from 3 independent experiments.

Article Snippet: p-JNK , Cell Signaling Technology , Cat#9255; RRID: AB_2307321.

Techniques: Western Blot, Expressing, Knockdown, Over Expression, Control